IWA 32:2019

This document provides requirements and recommendations to laboratories that perform genetically modified organism (GMO) analyses in cottonseed, leaf, cotton fibre and cotton fibre-derived materials.
The following are within the scope of this document:
a)    identifying the materials to be assessed, based on the probability of obtaining good quality, fit for purpose DNA from the materials in subsequent steps in the cotton cloth production process;
b)    specifying a method for efficient DNA isolation from cotton and cotton-derived materials described under point a);
c)    specifying the cotton-specific method(s) to be used as control for amplifiable DNA;
d)    specifying the screening procedure that provides optimal chances to detect GMOs as a result of the performance of the lowest number of genetically modified (GM) element screening assays.
NOTE 1    The protocol allows for the screening of all currently known GM cotton events and is set up in a way that optimizes the probability of also detecting unknown GM cotton events that possibly contain similar DNA sequences. Further information is given in CEN/TS 16707.
Sampling is outside of the scope of this document.
NOTE 2    A recommended sampling method is given in ISO 6497. General guidance for the sampling of bulk materials or for cotton-based products is available in standards such as ASTM D1441-12 and CEN/TS 15568.