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ISO 19040-1:2018

Water quality -- Determination of the estrogenic potential of water and waste water -- Part 1: Yeast estrogen screen (Saccharomyces cerevisiae)

General information

Valid from 07.08.2018
Directives or regulations
None

Standard history

Status
Date
Type
Name
07.08.2018
Main
This document specifies a method for the determination of the estrogenic potential of water and waste water by means of a reporter gene assay with genetically modified yeast strains Saccharomyces cerevisiae. This reporter gene assay is based on the activation of the human estrogen receptor alpha.
This method is applicable to:
-  fresh water;
-  waste water;
-  aqueous extracts and leachates;
-  eluates of sediments (fresh water);
-  pore water;
-  aqueous solutions of single substances or of chemical mixtures;
-  drinking water.
The limit of quantification (LOQ) of this method for the direct analysis of water samples is between 8 ng/l and 15 ng/l 17β-estradiol equivalents (EEQ) based on the results of the international interlaboratory trial (see Annex F). The upper threshold of the dynamic range for this test is between 120 ng/l and 160 ng/l 17β-estradiol equivalents (EEQ). Samples showing estrogenic potencies above this threshold have to be diluted for a valid quantification. Extraction and pre-concentration of water samples can prove necessary, if their estrogenic potential is below the given LOQ.

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