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EVS-ISO 16649-1:2011

Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 1: Colony-count technique at 44 degrees C using membranes and 5-bromo-4-chloro-3-indolyl beta-D-glucuronide (ISO 16649-1:2001)

General information

Withdrawn from 15.02.2019
Base Documents
ISO 16649-1:2001
Directives or regulations
None

Standard history

Status
Date
Type
Name
15.02.2019
Main
05.12.2011
Main
This part of ISO 16649 specifies a horizontal method for the enumeration of beta-glucuronidase-positive Escherichia
coli in products intended for human consumption or for the feeding of animals. It uses a colony-count technique
after resuscitation using membranes and incubation at 44 °C on a solid medium containing a chromogenic
ingredient for detection of the enzyme beta-glucuronidase.
WARNING — Strains of Escherichia coli which do not grow at 44 °C and, in particular, those that are
beta-glucuronidase negative, such as Escherichia coli O157, will not be detected.

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EVS-ISO 16649-2:2011

Microbiology of food and animal feeding stuffs - Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli - Part 2: Colony-count technique at 44 degrees C using 5-bromo-4-chloro-3-indolyl beta-D-glucuronide (ISO 16649-2:2001)
Newest version Valid from 05.12.2011