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EVS-EN ISO 15216-1:2017

Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 1: Method for quantification (ISO 15216-1:2017)

General information

Valid from 18.04.2017
Base Documents
ISO 15216-1:2017; EN ISO 15216-1:2017
Directives or regulations
None

Standard history

Status
Date
Type
Name
03.05.2021
Amendment
18.04.2017
Main
04.07.2013
Main
ISO 15216-1:2017 specifies a method for the quantification of levels of HAV and norovirus genogroup I (GI) and II (GII) RNA, from test samples of foodstuffs (soft fruit, leaf, stem and bulb vegetables, bottled water, BMS) or food surfaces. Following liberation of viruses from the test sample, viral RNA is then extracted by lysis with guanidine thiocyanate and adsorption on silica. Target sequences within the viral RNA are amplified and detected by real-time RT-PCR.
This method is not validated for detection of the target viruses in other foodstuffs (including multi-component foodstuffs), or any other matrices, nor for the detection of other viruses in foodstuffs, food surfaces or other matrices.

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EVS-EN ISO 15216-2:2019

Microbiology of the food chain - Horizontal method for determination of hepatitis A virus and norovirus using real-time RT-PCR - Part 2: Method for detection (ISO 15216-2:2019)
Newest version Valid from 01.10.2019